Natural Resistance against Lymphoma Grafts
نویسندگان
چکیده
Genetic factors are known to influence host resistance to certain tumors . In the mouse, resistance has been linked to the MHC locus on chromosome 17 (1-4) . H-2 genes or other closely linked genes may be responsible, and natural resistance to transplantable lymphomas represents one case where these two possibilities have not been resolved (5) . A distinct set of H-2-linked "hemopoietic histocompatibility" (Hh)' antigens has been postulated to explain the competence to reject small lymphoma grafts (5) . We have used the transgenic strain D8 (6) to analyze the genetic control of host resistance to tumors. The transgenic mouse D8 was generated by introducing an 8.0-kb genomic fragment containing the H-2Dd gene and 2 .5-kb 5' and 2.0-kb 3' flanking sequences into C57BL/6 (B6) zygotes (6). The transgene product was expressed in different tissues in the same way as the endogenous H-2b haplotype products, without alterations in the expression of the latter (6, 7) . The expressed antigen was also recognized as self, and could function as a restriction element for T cells (7) . We here report that the D8 mice have acquired "natural" resistance to C57BL lymphoma grafts in parallel with expression of the transgene product .
منابع مشابه
Natural resistance of lethally irradiated F1 hybrid mice to parental marrow grafts is a function of H-2/Hh-restricted effectors
The natural resistance of F1 hybrid mice against parental bone marrow grafts is thought to be mediated by natural killer (NK)-like effector cells. However, unlike the NK cell activity against a wide range of tumors and normal cells, hybrid resistance is characterized by the immunogenetic specificity controlled by a set of unique noncodominant genes denoted as Hh. Two alternative hypotheses can ...
متن کاملNatural resistance against lymphoma grafts conveyed by H-2Dd transgene to C57BL mice
The H-2Dd transgenic strain D8 on C57BL background was more resistant to subcutaneous challenge of RBL-5 lymphoma cells than B6 controls. The direct role of the H-2Dd antigen was investigated by the use of (D8 x B6)F1 crosses and (D8 B6) x B6 backcrosses. The latter showed cosegregation with regard to Dd antigen expression and lymphoma resistance, both of which were inherited in a pattern consi...
متن کاملNatural mechanisms of controlling lymphotropic herpesvirus infection (Marek's disease) in the chicken.
Four categories of natural resistance phenomena against Mareks disease in chickens, i.e., maternal antibody, age resistance, genetic resistance, and natural immunization, are described. In nature, these forms of resistance probably act in concert through comlex interrelationships. The mechanisms are not well understood but include humoral immunity (maternal antibody), cell-mediated immunity, an...
متن کاملHost resistance directed selectively against H-2-deficient lymphoma variants. Analysis of the mechanism
Three independent variants with a profound reduction of cell surface H-2 have been selected from the C57BL/6 mouse-derived RBL-5 and EL-4 T lymphomas. After subcutaneous inoculation of low cell doses in syngeneic mice, the H-2- variants failed to grow out, whereas the H-2+ control lines showed progressive growth. No difference in growth rate or cloning efficiency was detectable in tissue cultur...
متن کاملAfferent and efferent cellular interactions in natural resistance directed against MHC class I deficient tumor grafts.
H-2-deficient variants, selected from the murine lymphoma RBL-5, were recently shown to be less tumorigenic in syngeneic mice compared with the corresponding H-2-positive wild-type cell line. The present study focused on the detailed cellular interactions leading to the specific rejection of H-2-deficient cells in vivo. In vivo experiments with mixed tumor inocula, measuring either tumor outgro...
متن کامل